구강편평세포암종의 유세포분석적 DNA측정을 위한 이중 매개변수법의 적용
Application of dual parameter analysis in flow cytometric DNA measurements of oral squamous cell carcinoma
소속 상세정보
김수야
주훈/김재곤/조남표/백병주
KMID : 0358919960230020503
Abstract
series of 31 patients with primary oral squamous cell carcoma (SCC) who were treated at Chonbuk National University Hospital during the years 1991-1995, were evaluated by dual parameter analysis in flow cytometric DNA measurement, Bryne's
malignancy
grading system, and the TNM classification. The aims of the present study were to discover that previously undetected aneouploid clones could be detected by dual parameter analysis ad to determine the prognostic value of the above parameters.
1. Using dual parameter analysis of cytokeratin and DNA on disintegrated paraffin-embedded samples, aneuploid clones which were undetected by regular single parameter DNA-analysis could be found among the cytokeratin-selected cells. DNA
ancuploidy
from
paraffin-embedded samples were 15 cases compared with 10 cases using conventional DN?A analysis.
2. The portion of aneuploid tumors showed slightly higher clinical stage and tumor size than the portion of diploid tumors, but the differene was not significat. The portion of DNA aneuploid tumors showed significantly higher mean mitosis and
total
malignancy scores than the portion of DNA diploid tumors.
3. The majority of the patients presented with clinical stage ⅢandⅣlesions showed significantly higher mean total malignancy core as compared to those with clinical stage ⅠandⅡ.
4. Histopathologic mean total malignancy score of the 31 cases was 12.7. Among the histologic parameters, mean mitosis score was correlated to the status of DNA ploidy and total malignancy score were correlated to the DNA ploidy and clinical
staging.
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